Use of hematopoietic progenitor cell count on the Sysmex XE-2100 for peripheral blood stem cell harvest monitoring

The use of hematopoietic progenitor cell (HPC) count on the Sysmex XE-2100 for peripheral blood stem cell (PBSC) harvest monitoring is a valuable method in the field of hematopoietic stem cell transplantation. The Sysmex XE-2100 is an automated hematology analyzer that is widely used in clinical laboratories. Here is a detailed look at this application

Features:

• The Sysmex XE-2100 is a multiparameter hematology analyzer capable of performing a wide range of blood tests, including complete blood counts (CBCs) and differential counts.
• It uses fluorescence flow cytometry and semiconductor laser technology for accurate cell counting and differentiation.

HPC Counting:

• The XE-2100 can quantify HPCs by detecting cells that exhibit specific fluorescence characteristics.
• This capability provides a quick and reliable assessment of HPC levels in peripheral blood, facilitating timely and efficient PBSC harvesting.

Efficiency:

• Rapid processing and results allow for real-time monitoring of HPC levels.
• Reduces the need for manual counting and flow cytometry, which are more time-consuming and labor-intensive.

Accuracy:

• High sensitivity and specificity in detecting HPCs.
• Consistent and reproducible results improve the reliability of PBSC harvest timing.

Cost-Effectiveness:

• Reduces the costs associated with traditional HPC counting methods.
• Streamlines laboratory workflow, potentially lowering labor and operational expenses.

Non-Invasiveness:

• Regular monitoring can be done with routine blood samples, avoiding the need for more invasive procedures.

Monitoring and Harvesting:

• Patients undergoing stem cell mobilization therapy are monitored using the XE-2100 to determine the best time for PBSC collection.
• An optimal HPC count typically indicates a sufficient number of stem cells are in circulation, ready for collection.

Decision Making:

• Helps clinicians decide when to initiate apheresis (the process of collecting stem cells from the blood).
• Ensures that the harvested stem cell product contains an adequate number of HPCs for successful transplantation.

The Sysmex XE-2100 hematology analyzer’s capability to count hematopoietic progenitor cells in peripheral blood is a significant advancement in PBSC harvest monitoring. It provides a rapid, accurate, and cost-effective method for ensuring the collection of a sufficient number of stem cells, thus enhancing the success rates of stem cell transplantation procedures. By integrating this technology into clinical practice, healthcare providers can improve patient outcomes through more effective and efficient stem cell collection.